Raport științific - Etapa II 2019
Denumire etapă: Optimizarea protocolului de inverse PCR pentru cartarea situsurilor de inserție a unor IS
În cadrul acestei etape a fost optimizat protocolul de inverse PCR (iPCR) pentru cartarea inserțiilor transpozonilor bacterieni de tipul secvențelor de inserție (IS). A fost selectată ISKpn26 pentru optimizări, întrucât în etapa anterioară a fost identificată ca fiind una dintre cele mai frecvente IS din genomurile bacteriene analizate. Reacțiile de restricție și ligare enzimatică au fost optimizate pentru a oferi o cantitate optimă de matriță pentru amplificarea propriu-zisă a ADN circular. Au fost construiți și testați primeri specifici pentru iPCR. Rezultatele au evidențiat fenomene inserționale asociate cu rezistența la polimixine, tehnica de față oferind astfel o alternativă facilă de a detecta suportul genetic al rezistenței la această clasă de antibiotice, relativ greu de determinat prin alte metode fenotipice sau genotipice.
Scientific report - Phase II 2019
Phase title: iPCR protocol optimizing for mapping the insertion sites of some IS
During this phase, the inverse PCR (iPCR) protocol was optimised for mapping the insertion sites of the insertion sequences (IS). We selected the ISKpn26, as it was one of the most frequent encountered IS in the bacterial genomes analysed in the previous phase. The enzymatic restriction and ligation reactions were optimised in order to obtain an optimal quantity of reaction output. The iPCR primers were designed and tested. The results highlights the insertional background of polimixin resistance, thus offering an simple alternative to detect the genetic support of polomixin resistance, currently being relatively difficult to reveal using common phenotypic and genotypic methods.
Diseminarea rezultatelor /Results spreaing
Articol ISI publicat / Published ISI paper (BioMed Research International, IF = 2,20)
VC Cristea, I Gheorghe, I Czobor Barbu, ..., MC Chifiriuc, MI Popa. Snapshot of Phylogenetic Groups, Virulence, and Resistance Markers in Escherichia coli Uropathogenic Strains Isolated from Outpatients with Urinary Tract Infections in Bucharest, Romania. Biomed Res Int, 2019, 2019: Article ID 5712371
Articol ISI în evaluare / In evaluation ISI paper (Plos ONE, IF = 2,776)
M Surleac ¶, I Czobor Barbu ¶, S Paraschiv¶, ..., MC Chifiriuc. Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania, Plos ONE
Articol BDI publicat / Published BDI paper (Romanian Archives of Microbiology and Immunology)
LI Popa, I Czobor Barbu, MC Chifiriuc. Mobile Genetic Elements Involved in the Horizontal Transfer of Antibiotic Resistance Genes. Rom Arch Microb Immunol 2019; 77(4):274-287
Participare la conferința 29th European Congress of Clinical Microbiology and Infectious Diseases, Amsterdam, Olanda, 13-16 aprilie 2019
Participation in 29th European Conference of Clinical Microbiology and Infectious Diseases, Amsterdam, Netherlands, 13-16 April 2019
P0460 Detection of the epidemic IncP-6 plasmid carrying blaKPC-2 in a multidrugresistant Enterobcter kobei isolate from activated sludge of a wastewater treatment plant in Valcea County, Romania. I Czobor Barbu*, I Avram, I Gheorghe, LI Popa, AC Neguț, L Marutescu, M Popa, G Pircalabioru-Gradisteanu, V Lazar, MC Chifiriuc
P1226 Molecular characterisation of multidrug-resistant Klebsiella pneumoniae strains isolated from wastewater treatment plant in Romania
I Czobor Barbu, LI Popa, I Gheorghe, I Avram, AC Neguț, G Pircalabioru-Gradisteanu, V Lazar, MC Chifiriuc
P0936 Genetic background of antibiotic resistance in multidrug-resistant Acinetobacter baumannii strains recovered from a wastewater treatment plant near Bucharest, Romania. I Gheorghe*, I Czobor Barbu, I Avram, N Alshaikhli, LI Popa, M Popa, G Pircalabioru-Gradisteanu, AC Neguț, L Marutescu, V Lazar, MC Chifiriuc
Capitol de carte / Book chapter
Gene therapy: recent progress and perspectives, în Materials for Biomedical Engineering: Bioactive Materials for Antimicrobial, Anticancer, and Gene Therapy, Chapter 10, pages 283-295, Elsevier, 2019